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Antibodies bound to the blocking peptide now not bind on the epitope around the concentrate on protein. By comparing the staining from the blocked antibody as opposed to the antibody alone, you can see which staining is specific.
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This guidebook outlines further more achievable optimization of mobile and immunoassay parameters to be sure the absolute best results are...
This guidebook outlines further achievable optimization of cellular and immunoassay parameters to guarantee the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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Blocking peptides are peptides that bind precisely into the focus on antibody and block antibody binding. These peptide typically includes the epitope recognized via the antibody.
Focus on Data Cyclin E here belongs for the remarkably conserved cyclin loved ones, whose associates are characterized by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
Overexpression of the gene has actually been observed in many tumors, which ends up in chromosome instability, and so may well add to tumorigenesis. This protein was located to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical position in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two added splice variants were being reported but detailed nucleotide sequence info just isn't nonetheless available.
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